Dot Blot:Article Title: Supplemental Information Long noncodingRNALnc530 localizes onR-loops and regulatesR-loop formation and genomic stability in mouse embryonic stem cells
Article Snippet: .. Antibodies Source Dilution DDX5 Rabbit monoclonal antibody Abcam; Cat# ab126730 1:1000 (IF) 1:1000 (IB) 1:50 (IP) TDP-43 Rabbit monoclonal antibody Abcam; Cat# ab190963 1:200 (IF) 1:1000 (IB) 1:50 (IP) S9.6 Mouse monoclonal antibody Kerafast; Cat# ENH001 1:100 (IF) S9.6 Mouse monoclonal antibody ATCC; Cat# HB-8730 1:50 (IP) 1:500 (Dot blot) dsDNA Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-58749 1:500 (Dot blot) BrdU Rat monoclonal antibody, also used for immunostaining of CIdU Abcam; Cat# ab6326 1:1000 (IF) γH2AX Rabbit polyclonal antibody Cell Signaling Technology; Cat# 9718 1:1000 (IB) 1:1000 (IF) RNase H1 Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-376326 1:500 (IB) GAPDH Mouse monoclonal antibody Sangon Biotech; Cat# D190090 1:1000 (IB) Flag Rabbit polyclonal antibody Sigma; Cat# F7425 1:1000 (IB) SOX2 Rabbit polyclonal antibody Merck Millipore; Cat# AB5603 1:500 (IB) NANOG Rabbit polyclonal antibody Abcam; Cat# ab70482 1:1000 (IB) OCT4 Rabbit polyclonal antibody Abcam; Cat# ab19857 1:1000 (IB) Fibrillarin Rabbit monoclonal antibody Cell Signaling Technology; Cat# 2639 1:1000 (IF) Histone H3 Mouse monoclonal antibody TransGen Biotech; Cat# HL102 1:1000 (IB) ACTB Mouse monoclonal antibody Abclonal; Cat# AC004 1:1000 (IB) Goat anti-Rat IgG secondary antibody, Alexa Fluor Cy3 Thermo Fisher Scientific; Cat# A-10522 1:500 (IF) Goat anti-Mouse IgG (H+L) secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11029 1:500 (IF) Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31570 1:500 (IF) Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11034 1:500 (IF) Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31572 1:500 (IF) Goat anti-Mouse IgG (H+L) Thermo Fisher Scientific; 1:5000 (IB) secondary antibody, HRP Cat# 31430 Goat anti-Rabbit IgG (H+L) secondary antibody, HRP Thermo Fisher Scientific; Cat# 31460 1:5000 (IB) 5-Chloro-2′-deoxyuridine (CIdU) Sigma-Aldrich; Cat# C6891 20 μM Hydroxylurea (HU) Sigma-Aldrich; Cat# H8627 4 mM 5,6-Dichlorobenzimidazole 1-β-DRibofuranoside Tokyo Chemical Industry; Cat# ; D4292 10 μM ..
Article Title: Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells
Article Snippet: .. Antibodies Source Dilution DDX5 Rabbit monoclonal antibody Abcam; Cat# ab126730 1:1000 (IF) 1:1000 (IB) 1:50 (IP) TDP-43 Rabbit monoclonal antibody Abcam; Cat# ab190963 1:200 (IF) 1:1000 (IB) 1:50 (IP) S9.6 Mouse monoclonal antibody Kerafast; Cat# ENH001 1:100 (IF) S9.6 Mouse monoclonal antibody ATCC; Cat# HB-8730 1:50 (IP) 1:500 (Dot blot) dsDNA Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-58749 1:500 (Dot blot) BrdU Rat monoclonal antibody, also used for immunostaining of CIdU Abcam; Cat# ab6326 1:1000 (IF) γH2AX Rabbit polyclonal antibody Cell Signaling Technology; Cat# 9718 1:1000 (IB) 1:1000 (IF) RNase H1 Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-376326 1:500 (IB) GAPDH Mouse monoclonal antibody Sangon Biotech; Cat# D190090 1:1000 (IB) Flag Rabbit polyclonal antibody Sigma; Cat# F7425 1:1000 (IB) SOX2 Rabbit polyclonal antibody Merck Millipore; Cat# AB5603 1:500 (IB) NANOG Rabbit polyclonal antibody Abcam; Cat# ab70482 1:1000 (IB) OCT4 Rabbit polyclonal antibody Abcam; Cat# ab19857 1:1000 (IB) Fibrillarin Rabbit monoclonal antibody Cell Signaling Technology; Cat# 2639 1:1000 (IF) Histone H3 Mouse monoclonal antibody TransGen Biotech; Cat# HL102 1:1000 (IB) ACTB Mouse monoclonal antibody Abclonal; Cat# AC004 1:1000 (IB) Goat anti-Rat IgG secondary antibody, Alexa Fluor Cy3 Thermo Fisher Scientific; Cat# A-10522 1:500 (IF) Goat anti-Mouse IgG (H+L) secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11029 1:500 (IF) Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31570 1:500 (IF) Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11034 1:500 (IF) Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31572 1:500 (IF) Goat anti-Mouse IgG (H+L) Thermo Fisher Scientific; 1:5000 (IB) secondary antibody, HRP Cat# 31430 Goat anti-Rabbit IgG (H+L) secondary antibody, HRP Thermo Fisher Scientific; Cat# 31460 1:5000 (IB) 5-Chloro-2′-deoxyuridine (CIdU) Sigma-Aldrich; Cat# C6891 20 μM Hydroxylurea (HU) Sigma-Aldrich; Cat# H8627 4 mM 5,6-Dichlorobenzimidazole 1-β-D- Ribofuranoside Tokyo Chemical Industry; Cat# ; D4292 10 μM ..
Immunostaining:Article Title: Supplemental Information Long noncodingRNALnc530 localizes onR-loops and regulatesR-loop formation and genomic stability in mouse embryonic stem cells
Article Snippet: .. Antibodies Source Dilution DDX5 Rabbit monoclonal antibody Abcam; Cat# ab126730 1:1000 (IF) 1:1000 (IB) 1:50 (IP) TDP-43 Rabbit monoclonal antibody Abcam; Cat# ab190963 1:200 (IF) 1:1000 (IB) 1:50 (IP) S9.6 Mouse monoclonal antibody Kerafast; Cat# ENH001 1:100 (IF) S9.6 Mouse monoclonal antibody ATCC; Cat# HB-8730 1:50 (IP) 1:500 (Dot blot) dsDNA Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-58749 1:500 (Dot blot) BrdU Rat monoclonal antibody, also used for immunostaining of CIdU Abcam; Cat# ab6326 1:1000 (IF) γH2AX Rabbit polyclonal antibody Cell Signaling Technology; Cat# 9718 1:1000 (IB) 1:1000 (IF) RNase H1 Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-376326 1:500 (IB) GAPDH Mouse monoclonal antibody Sangon Biotech; Cat# D190090 1:1000 (IB) Flag Rabbit polyclonal antibody Sigma; Cat# F7425 1:1000 (IB) SOX2 Rabbit polyclonal antibody Merck Millipore; Cat# AB5603 1:500 (IB) NANOG Rabbit polyclonal antibody Abcam; Cat# ab70482 1:1000 (IB) OCT4 Rabbit polyclonal antibody Abcam; Cat# ab19857 1:1000 (IB) Fibrillarin Rabbit monoclonal antibody Cell Signaling Technology; Cat# 2639 1:1000 (IF) Histone H3 Mouse monoclonal antibody TransGen Biotech; Cat# HL102 1:1000 (IB) ACTB Mouse monoclonal antibody Abclonal; Cat# AC004 1:1000 (IB) Goat anti-Rat IgG secondary antibody, Alexa Fluor Cy3 Thermo Fisher Scientific; Cat# A-10522 1:500 (IF) Goat anti-Mouse IgG (H+L) secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11029 1:500 (IF) Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31570 1:500 (IF) Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11034 1:500 (IF) Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31572 1:500 (IF) Goat anti-Mouse IgG (H+L) Thermo Fisher Scientific; 1:5000 (IB) secondary antibody, HRP Cat# 31430 Goat anti-Rabbit IgG (H+L) secondary antibody, HRP Thermo Fisher Scientific; Cat# 31460 1:5000 (IB) 5-Chloro-2′-deoxyuridine (CIdU) Sigma-Aldrich; Cat# C6891 20 μM Hydroxylurea (HU) Sigma-Aldrich; Cat# H8627 4 mM 5,6-Dichlorobenzimidazole 1-β-DRibofuranoside Tokyo Chemical Industry; Cat# ; D4292 10 μM ..
Article Title: Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells
Article Snippet: .. Antibodies Source Dilution DDX5 Rabbit monoclonal antibody Abcam; Cat# ab126730 1:1000 (IF) 1:1000 (IB) 1:50 (IP) TDP-43 Rabbit monoclonal antibody Abcam; Cat# ab190963 1:200 (IF) 1:1000 (IB) 1:50 (IP) S9.6 Mouse monoclonal antibody Kerafast; Cat# ENH001 1:100 (IF) S9.6 Mouse monoclonal antibody ATCC; Cat# HB-8730 1:50 (IP) 1:500 (Dot blot) dsDNA Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-58749 1:500 (Dot blot) BrdU Rat monoclonal antibody, also used for immunostaining of CIdU Abcam; Cat# ab6326 1:1000 (IF) γH2AX Rabbit polyclonal antibody Cell Signaling Technology; Cat# 9718 1:1000 (IB) 1:1000 (IF) RNase H1 Mouse monoclonal antibody Santa Crus Biotechnology; Cat# sc-376326 1:500 (IB) GAPDH Mouse monoclonal antibody Sangon Biotech; Cat# D190090 1:1000 (IB) Flag Rabbit polyclonal antibody Sigma; Cat# F7425 1:1000 (IB) SOX2 Rabbit polyclonal antibody Merck Millipore; Cat# AB5603 1:500 (IB) NANOG Rabbit polyclonal antibody Abcam; Cat# ab70482 1:1000 (IB) OCT4 Rabbit polyclonal antibody Abcam; Cat# ab19857 1:1000 (IB) Fibrillarin Rabbit monoclonal antibody Cell Signaling Technology; Cat# 2639 1:1000 (IF) Histone H3 Mouse monoclonal antibody TransGen Biotech; Cat# HL102 1:1000 (IB) ACTB Mouse monoclonal antibody Abclonal; Cat# AC004 1:1000 (IB) Goat anti-Rat IgG secondary antibody, Alexa Fluor Cy3 Thermo Fisher Scientific; Cat# A-10522 1:500 (IF) Goat anti-Mouse IgG (H+L) secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11029 1:500 (IF) Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31570 1:500 (IF) Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific; Cat# A-11034 1:500 (IF) Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 Thermo Fisher Scientific; Cat# A-31572 1:500 (IF) Goat anti-Mouse IgG (H+L) Thermo Fisher Scientific; 1:5000 (IB) secondary antibody, HRP Cat# 31430 Goat anti-Rabbit IgG (H+L) secondary antibody, HRP Thermo Fisher Scientific; Cat# 31460 1:5000 (IB) 5-Chloro-2′-deoxyuridine (CIdU) Sigma-Aldrich; Cat# C6891 20 μM Hydroxylurea (HU) Sigma-Aldrich; Cat# H8627 4 mM 5,6-Dichlorobenzimidazole 1-β-D- Ribofuranoside Tokyo Chemical Industry; Cat# ; D4292 10 μM ..
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